2003 US Frontiers of Science Symposium

2005 US Frontiers of Science Symposium

Small RNAs

Phillip D. Zamore, University of Massachusetts Medical School

1. Introduction

2. RNAi: dsRNA-targeted gene silencing

3. RNAi: a tool for inhibiting gene expression in vivo

5. What are the biological functions of RNAi

21. Antisense RNA : RNA pairing: 21 + 21 nt

22. Small RNA: target RNA pairing: ~7nt + 21nt

25. RNase III endonucleases and their dsRBD partner proteins

26. RNase III endonucleases and their dsRBD partner proteins

27. RNase III endonucleases and their sdRBD partner proteins

28. RNase III endonucleases and their sdRBD partner proteins

29. Loqsf00791: strong germ-line, but mild somatic, allele

30. Pre-miRNA maturation requires both Dcr-1 and Loqs

31. A YFP reporter repressed by a miRNA in vivo

32. miRNA function in vivo requires Loqs

33. miRNA function in vivo requires Loqs, but not R2D2

34. miRNA function in vivo requires Loqs, but not R2D2

35. Loqs mutants make very few oocytes

36. Loqs mutant ovaries do not maintain germ line stem cells

37. Loqs females die young

38. Loqs males also die young

39. Loqs females die even younger when mated

40. .. But loqs males die no younger when mated

41. Acknowledgements

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Copyright © 2005. National Academy of Sciences. All rights reserved.